![]() Each figure should include all relevant controls, and when relevant, control samples should be run on the same blot or gel as the experimental samples.When a comparative analysis amongst bands is performed, all relevant samples should be run on the same gel/blot.Any non-specific bands from the original image should be included in the figure and explained in the text or figure legend. Figure panels should include some background area above and below bands. Images in figures should not be overcropped around the bands of interest.Any adjustments to improve visibility of the data must be consistently applied to the entire image. The background and contrast should not be modified to notably alter the visibility of data, background, or non-specific bands. ![]() In accordance with our Policy on Figure Preparation, the images should not be adjusted in any way that could affect the scientific information displayed. ![]() Reporting blot and gel resultsĪuthors must comply with the following guidelines when preparing figures that report results from blots (including, but not limited to, western blots) and electrophoretic gels: If concerns are noted after publication, journal staff will follow up with authors and correct the literature as needed. A manuscript must comply with the above requirements, and the guidelines below, in order to be published. If any concerns arise about the availability of the original blot or gel images or compliance with the figure preparation guidelines (see below), the submission will be placed on hold while this is resolved. If your PDF size is larger, please use a suitable repository or discuss with the journal staff.
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